Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils

Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils

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Summary: Polymorphonuclear neutrophils (PMNs), the most abundant white blood cells, are recruited rapidly to sites of infection to exert potent anti-microbial activity.Information regarding their role in infection with human immunodeficiency virus (HIV) is limited.Here we report that addition of PMNs to HIV-infected cultures of human tonsil tissue or peripheral popularfilm.blog blood mononuclear cells causes immediate and long-lasting suppression of HIV-1 spread and virus-induced depletion of CD4 T cells.This inhibition of HIV-1 spread strictly requires PMN contact with infected cells and is not mediated by soluble factors.2-Photon (2PM) imaging visualized contacts of PMNs with HIV-1-infected CD4 T cells in tonsil tissue that do not result in lysis or uptake of infected cells.

The anti-HIV activity of PMNs also does not involve degranulation, formation of neutrophil extracellular traps, or knowall.blog integrin-dependent cell communication.These results reveal that PMNs efficiently blunt HIV-1 replication in primary target cells and tissue by an unconventional mechanism.